Bio-Tools

FASTA to Phylip Converter

Bioinformatics Converter

Convert between various bioinformatics formats instantly

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Authors: Mahdi Morshedi Yekta, Fatemeh Faryadras | Contact Support

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Our easy-to-use web-based converter instantly converts your aligned FASTA sequences into PHYLIP format. The PHYLIP format is essential for many phylogenetic analysis tools, such as PHYLIP, RAxML, and PHYML. Our tool ensures accurate transformation of multiple sequence alignments from FASTA to PHYLIP.

🔄 How to Use:

  1. Upload your aligned FASTA file (.fasta or .fa).
  2. Select PHYLIP format type (sequential or interleaved) if available.
  3. Click “Convert to PHYLIP”.
  4. Download your ready-to-use .phy file.

🧬 Why Convert to PHYLIP Format?

The PHYLIP format is widely used for input in many phylogenetic software packages. It requires sequences to be aligned and conform to a strict structure. Converting from FASTA ensures:

  • Compatibility with tree-building tools like PHYLIP, RAxML, FastTree
  • Standardized input for maximum reproducibility
  • Simplified integration into bioinformatics pipelines

🔍 Example FASTA Input:

>SeqA
ATGCTAGCTAGCTA
>SeqB
ATGCTAGCTAGTTA

🔍 Converted PHYLIP Output (Sequential):

 2 14
SeqA      ATGCTAGCTAGCTA
SeqB      ATGCTAGCTAGTTA

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